Certificate of Analysis(ver.1.0)

Aflatoxin B₁ in Acetonitrile

1.General information

This document is designed and the certified value(s) and uncertainty(ies) are determined in accordance with ISO Guide 31^[1]

2. Description of the Reference Material(RM)

Product name: Aflatoxin B in Acetonitrile 
Product number: STD#1045 
CAS number: 1162-65-8 
Formula: C₁₇H₁₂O₆ 
Formula weight: 312.27 
Lot#: 2A00G19 
Result concentration: 20.05±0.28μg/mL 
Starting material: Aflatoxin B,lot#J20016P,Pribolab 
Amount: 1.2mL 
Matrix: Acetonitrile,LiChrosolv,Merck 
Prcduction date: 19,Jul,2021 
Expiry date: 18Jan,2023 
Name of the supplier: Pribolab Pte.Ltd.

2.1 Intended use of the RM

-for laboratory use only
- calibration of analytical instruments

2.2 Instruction for the correct use of the RM

The compound should be stored at -20℃ or below in a dark place.Before usage of the RMthe compound should be allowed to warm to temperature(20±3℃).The recommended minimum sub-sample amount for all kinds of application is 100 uL.The expiry date of this RM is based on the current knowledge and holds only for proper storage conditions in the originally closed flasks/ packages.

2.3 Hazardous situation

The normal laboratory safety precautions should be observed when working with this RM.Further details for the handing of this RM are available as safety data sheet

3. Certified values and their uncertainties

3.1 Calculation of uncertainty

After the concentration of the gravimetric prepared solution was confirmed by HPLC-DAD,the uncertainty of the calibrant was calculated on the basis of preparation^[3]

Uncertainty components	Description Standard	uncertainty(u)
Purity(P)of solid Aflatoxin B1	P=99.0±1.0%	u(P)=0.6%	a
Weighing procedure Weighted sample:Mws=2.025mg	U(m)=0.0000008g+1.30*10-5*m Toxin U(m)=U(m)/2	u(m)=0.0004mg	b
Dilution procedure Volumetric flask:v1=100mL	Calibration:100mL±0.1mL Repeatability:0.04mL Volume expansion solvent	u(cal)=0.04mL u(rep)=0.04mL u(Vol.exp.1)=0.24mL u(v)=0.25mL	c d e f
Calculation of the combined uncertainty uc and the expanded standard uncertainty U

4.Discussion of traceability

This calibrant is certified on the basis of gravimetric preparation4.Thus the certified value(mass concentration of Aflatoxin B1is based on the weighed amount of the starting material and is therefore traceable to the stated purity of the solid raw material.High purity material represents a practical realization of concentration units,through conversion of mass to molar quantity

5. Confirmation of certified value by HPLC-DAD

The certified concentration of Aflatoxin B₁ of the gravimetric prepared solution was confirmed bv HPLC-DAD against an independently prepared reference batch of Aflatoxin B₁.

6.Further information

The purchaser must determine the suitability of this product for its particular use. Pribolab makes no warranty of any kind,express or implied,other than its products meet all quality control standards set by Pribolab.We do not guarantee that the product can be used for a special application

References:

[1] ISO Guide 31,1-7,(2000),“Reference Materials-Contents of Certificates and Labels
[2] International Organization for Standardization(ISO)，(2008),“Guide to the Expression of Uncertainty in Measurements”,(GUM 1995 with minor corrections)1stEdGeneva,Switzerland
[3]R.D.Josephs,R.Krska,S.MacDonald,P.Wilson,H.PetterssonJ.AOACInt.86,50-60(2003) “Preparation of a Calibrant as Certified Reference Material for Determination of the Fusarium Mycotoxin,Zearalenone”
[4]EW.Flick,(1998),“Industrial Solvents Handbook”,5rEd.Noyes Data Corp.Westwood NJ

注：以上信息仅供参考，以产品附带证书为准。